Revision as of 06:30, 23 September 2010

UT-Tokyo

Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent

@@ Line 13: / Line 13: @@
-Transformation
+[[Transformation]]
-Colony Pick Up
-Miniprep
+[[Colony Pick Up]]
-Restriction Enzyme Transaction（Xba1/Pst1）—Once for all
+[[Miniprep]]
-Restriction Enzyme Transaction —One by one
+[[Restriction Enzyme digestion（Xba1/Pst1）—Once for all]]
-PCR（Pfu　Ultra）
+[[Restriction Enzyme digestion —One by one]]
-PCR（Ex-Taq）
+[[PCR（Pfu　Ultra）]]
-PCR（KODplus）
+[[PCR（Ex-Taq）]]
-Colony PCR（Ex-Taq）
+[[PCR（KODplus）]]
-Electrophoresis
+[[Colony PCR（Ex-Taq）]]
-Gel DNA Recovery
+[[Electrophoresis]]
-LB　broth
+[[Gel DNA Recovery]]
-Agarose Gel
+[[LB　broth]]
-Seaquencing
+[[Agarose Gel]]
-Producing Competent Cell
+[[Seaquencing]]
+[[Producing Competent Cell]]
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