Team:UT-Tokyo/Gel DNA Recovery

From 2010.igem.org

UT-Tokyo

Gel DNA Recovery

Preparation

  • ・Kit of promega
  • ・MilliQ
  • ・Gel
  • ・Make sure that tank for electrophoresis and EtBr are not polluted.

Procedure

  • 1. Cut out gel with wanted band and put it in a tube.
  • 2. Add 3 parts Mem. binding sol. to 1 part Gel volume.
  • 3. Shake & Incubate at 57 degrees C until gel melts.
  • 4. Put in the column.
  • 5. Centrifuge for 1 min at the largest spin number (at room temperature)
  • 6. Empty collection tube and add 700 ul Mem. Wash sol, centrifuge for 1 minute.
  • 7. Empty collection tube and add 500 ul Mem. Wash sol, centrifuge for 1 minute.
  • 8. Change the column to a new 1.5 ml Eppendorf and centrifuge for 1 minute.
  • 9. Change the old tube to a new 1.5 ml Eppendorf, add 30ul MilliQ, leave for 1 minutes, then centrifuge for 1 minutes.
    • ! Use Nuclease-Free Water instead of MilliQ
  • 10. Measure concentration, label the Eppendorf.