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Team:UT-Tokyo/Electrophoresis
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Latest revision as of 08:21, 23 September 2010
Electrophoresis
Preparation
- ・Sample
- ・Gel （1% gel for fine separation, 2% for rough separation, use 2 % gel for colony PCR)
- ・DNA loading dye
- ・DNA λH200 ladder mix
Procedure
- 1. Add 1 ul dye for 10 ul sample, and apply to the well.
- 2. Apply 2 ul ladder to the edge well, electrophoresis for 30 minutes.
- ! DNA chain fewer than 100 bp has danger of over flowing.If you do electrophoresis for 27 minutes, chain of 129 bp will narrowly stay in the gel.
- 3. Dye with EtBr for about 20 minutes.
- 4. Observe by UV.
- Use “Fujifilm” to cut out. This one needs time to boot-up, so turn on the switch first.
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