Team:UT-Tokyo/Colony Pick Up

From 2010.igem.org

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(New page: __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} Preparation *test tube *LBbroth 4ml/tube *antibiotics (take out from fridge and use after thawing out / needless to use after colony PCR) Pro...)
 
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__NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}}
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<h1>Colony Pick Up</h1>
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Preparation
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<h2>Preparation</h2>
*test tube  
*test tube  
*LBbroth 4ml/tube
*LBbroth 4ml/tube
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(take out from fridge and use after thawing out / needless to use after colony PCR)
(take out from fridge and use after thawing out / needless to use after colony PCR)
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Procedure
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<h2>Procedure</h2>
*1. add 2 μm of LB broth to the tube  
*1. add 2 μm of LB broth to the tube  
*2. add the antibiotic to the tube(1μm for 1ml)
*2. add the antibiotic to the tube(1μm for 1ml)

Latest revision as of 07:24, 23 September 2010

UT-Tokyo

Colony Pick Up

Preparation

  • test tube
  • LBbroth 4ml/tube
  • antibiotics

(take out from fridge and use after thawing out / needless to use after colony PCR)

Procedure

  • 1. add 2 μm of LB broth to the tube
  • 2. add the antibiotic to the tube(1μm for 1ml)
  • 3. poke the colony on the plate by toothpick(or tip of pipette)and put the toothpick to the tube
  • 4. label the cap of the tube and fix the cap on the tube
  • 5. incubate it at 37 degrees (shake culture)

(15～18 hours is best / 8 hours is the fastest)

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