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Team:USTC Software/Toggle-Switch
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(New page: ==Toggle Switch== The toggle switch is composed of two repressors and two promoters, each of which is inhibited by the repressor transcribed by the other promoter. The parts assembling is ...) |
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| - | The toggle switch is composed of two repressors and two promoters, each of which is inhibited by the repressor transcribed by the other promoter. The parts assembling is | + | [[Image:USTCs Toggle assembly.PNG|450px|thumb|right|The top assembling is the construction used in our software; The bottom one is the toggle switch design in [http://www.nature.com/nature/journal/v403/n6767/full/403339a0.html Construction of a genetic toggle switch in Escherichia coli] ]] |
| + | The toggle switch is composed of two repressors and two promoters, each of which is inhibited by the repressor transcribed by the other promoter. The parts assembling is shown in the figure. We choose pLacI and pTetR as the two promoters, and the repressors are TetR and LacI, respectively. Theoretically, if ratio of LacI to TetR is greater than 1, strong binding of LacI to pLacI repress transcription initiation from pLacI and therefore quantities of TetR will decrease, which in turn relieves its repression to pTet leading to increase of quantities of LacI itself. In this situation, concentration of LacI is far greater than that of tetR, whose expression is repressed at an extream low level and the system enters into its 'LacI' state. Similar analysis will do for TetR and the system will enter into 'TetR' state if its quantity dominates. To show transition between the two states, IPTG is added in 1s after the system enters into 'LacI' state completely. | ||
Revision as of 00:18, 26 October 2010
Toggle Switch
The toggle switch is composed of two repressors and two promoters, each of which is inhibited by the repressor transcribed by the other promoter. The parts assembling is shown in the figure. We choose pLacI and pTetR as the two promoters, and the repressors are TetR and LacI, respectively. Theoretically, if ratio of LacI to TetR is greater than 1, strong binding of LacI to pLacI repress transcription initiation from pLacI and therefore quantities of TetR will decrease, which in turn relieves its repression to pTet leading to increase of quantities of LacI itself. In this situation, concentration of LacI is far greater than that of tetR, whose expression is repressed at an extream low level and the system enters into its 'LacI' state. Similar analysis will do for TetR and the system will enter into 'TetR' state if its quantity dominates. To show transition between the two states, IPTG is added in 1s after the system enters into 'LacI' state completely.
