Team:USTC/Modeling/a

From 2010.igem.org

Revision as of 01:42, 25 October 2010 by Jocelynatustc (Talk | contribs)

An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles

Known Conditions

This table illustrate some basic information of pdu shell protein family.[http://www.ncbi.nlm.nih.gov/pubmed/20417607]


USTC2010_known_conditions_table_1.jpg

Table 1| Basic information of pdu shell protein family.


USTC2010_known_conditions_figure_1.jpg

Figure 1| 3-dimension structure of the known shell proteins from Salmonella enterica . UPPER: pduU. [http://www.ncbi.nlm.nih.gov/pubmed/18786396] MIDDLE: pduA. [http://www.ncbi.nlm.nih.gov/pubmed/20870711] DOWN:pduT. [http://www.ncbi.nlm.nih.gov/pubmed/20870711 [3]]

Hypothesis

I)Pdu N is the vertex and its pentametic structuresfor its identity with a proved vertex pentametic protein---CcmL. [http://www.ncbi.nlm.nih.gov/pubmed/18292340]

II) Hexamers forms the facets, which comprises the building blocks of bacterial microcompartment shells. [http://www.ncbi.nlm.nih.gov/pubmed/17518518]

USTC2010_hypothesis_N_vertex.jpg USTC2010_Hypothesis_Hexamer.jpg

Figure 2| LEFT: A diagram showing the construction of a large icosahedron from many smaller hexagons and 12 pentagons at the vertices. The figure shown has a triangulation number (T) of 75 (29). RIGHT: Individual CsoS1A molecules, typical shell proteins, are differently in each hexamer.

III) Similarity of the shell proteins to the carboxysome [http://www.ncbi.nlm.nih.gov/pubmed/12923081] and carboxysome is believed to be in icosohedral shape.

Results

Conclusion

Reference

[1] Martin J. Warren, et al. 2010. Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement. Molecular Cell. 2010 Apr 38 (2), 305–315

[2] Structure of the PduU shell protein from the Pdu microcompartment of Salmonella. Structure. 2008 Sep 16(9):1324-32

[3] Structural insights into the mechanisms of transport across the Salmonella enterica Pdu microcompartment shell. J Biol Chem. 2010 Sep: [Epub ahead of print]

[4] Atomic-level models of the bacterial carboxysome shell. Science. 2008 Feb, 319(5866):1083-6

[5] Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome. PLoS Biol. 2007 Jun;5(6):e144.

[6] Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar Typhimurium LT2. J Bacteriol. 2003 Sep;185(17):5086-95