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Team:UPO-Sevilla/Notebook/10 11
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<h1>October, 11th</h1> | <h1>October, 11th</h1> | ||
| - | <h2> | + | <h2>Assembly Team</h2> |
| - | <p>Minipreps of 1+2+7+3 different inocula and digestion of them (2h, 37ºC). | + | <p>Minipreps of 1+2+7+3 different inocula and digestion of them (2h, 37ºC). 22 samples were run in an agarose gel. Results were negative. This device has a problem: either it is not viable or 1+2 quantity is not enough. Other UPO 1+2 preparation was made but using more DNA.</p> |
<p>Ligation of 12+2+13+3 (3A and SA). | <p>Ligation of 12+2+13+3 (3A and SA). | ||
</p> | </p> | ||
Latest revision as of 15:28, 27 October 2010
October, 11th
Assembly Team
Minipreps of 1+2+7+3 different inocula and digestion of them (2h, 37ºC). 22 samples were run in an agarose gel. Results were negative. This device has a problem: either it is not viable or 1+2 quantity is not enough. Other UPO 1+2 preparation was made but using more DNA.
Ligation of 12+2+13+3 (3A and SA).
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