August, 3rd

Production Team

Paola Gallardo. We digest fecI-fecR with PstI and EcoRI restriction enzymes , and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).

Assembly Team

We analyzed several candidates from 2+4, 2+5 and 2+7 plates by colony PCR. We had positive candidates of UPO2+4 and UPO2+5. We set up inocula of these candidates and spread on LB+Tc plates.