Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test28settembre

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Revision as of 22:33, 14 October 2010

23° TEST

[SEPTEMBER, 28TH]

PLATE

EXPERIMENT DESCRIPTION

Motivation

This experiment was performed to check bacterial growth and GFP rate synthesis of the following constructs in order to verify right protein folding and efficiency of inducible system assembled upstream of some genetic circuits.

Methods

Inoculum (into 5 ml LB+Amp) from glycerol stock of:

I63
I64
I65
<partinfo>BBa_K173002</partinfo> (positive control)
<partinfo>BBa_B0031</partinfo> (negative control)

They were let grow ON at +37°C, 220 rpm.

The following day cultures were diluted 1:100 and let grow again for about five hours at +37°C, 220 rpm.

Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02.

Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced HSL 100nM directly in multiplate well. Each value shown is the mean of three measurements; cultures were shaked for 15 seconds every five minutes.

RESULTS (?)

MEASUREMENTS

Tecan Test

@@ Line 18: / Line 18: @@
 ==EXPERIMENT DESCRIPTION==
+===Motivation===
+This experiment was performed to check bacterial growth and GFP rate synthesis of the following constructs in order to verify right protein folding and efficiency of inducible system assembled upstream of some genetic circuits.
+===Methods===
+Inoculum (into 5 ml LB+Amp) from glycerol stock of:
+*I63
+*I64
+*I65
+*<partinfo>BBa_K173002</partinfo> (positive control)
+*<partinfo>BBa_B0031</partinfo> (negative control)
+They were let grow ON at +37°C, 220 rpm.
+The following day cultures were diluted 1:100 and let grow again for about five hours at +37°C, 220 rpm.
+Optical density (O.D.) of each cell culture was than measured with TECAN Infinte F200. Samples were diluted in order to obtain the same O.D. equal to 0,02.
+Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced HSL 100nM directly in multiplate well.  Each value shown is the mean of three measurements; cultures were shaked for 15 seconds every five minutes.
 ==RESULTS (?)==