Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27settembre
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[[Image:UNIPV_Pavia_SScellM27settembre.png|thumb|500px |center|Mean of (dGFP/dt)/O.D.600 (under the hypothesis that GFP half-life in fusion contructs is similar to the original one - <partinfo>BBa_E0040</partinfo>)]] | [[Image:UNIPV_Pavia_SScellM27settembre.png|thumb|500px |center|Mean of (dGFP/dt)/O.D.600 (under the hypothesis that GFP half-life in fusion contructs is similar to the original one - <partinfo>BBa_E0040</partinfo>)]] | ||
- | All cell cultures showed a similar growth curve | + | All cell cultures showed a similar growth curve; doubling time was computed as described [[Team:UNIPV-Pavia/Parts/Characterization#Doubling_time_evaluation|here]] in order to have informations about the burden due to synthesis of such fusion proteins. It's possible to see that all doubling time are very similar; it's possible to assert that the expression of these BioBrick parts doesn't cause abnormal stress to cells. |
From GFP curve it's possible to appreciate that in I47, I48, I49 GFP accumulation it's very similar and it's significantly different from that of negative control <partinfo>BBa_B0031</partinfo>. This result that the green fluorescent protein assembled downstream of the genetic circuit is correctly folded. | From GFP curve it's possible to appreciate that in I47, I48, I49 GFP accumulation it's very similar and it's significantly different from that of negative control <partinfo>BBa_B0031</partinfo>. This result that the green fluorescent protein assembled downstream of the genetic circuit is correctly folded. |
Revision as of 19:21, 27 October 2010
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