Team:UNIPV-Pavia/Calendar/October/settimana3

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OCTOBER: WEEK 3



October, 18th

We have finally completed the parts transfer to <partinfo>pSB1C3</partinfo> plasmid. All the parts that will be sent to the registry were previously screened by UNIPV-Pavia TEAM, as reported in this notebook and all of them were sequenced and sequencing results were correct.

After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis.

The parts we sent to MIT are:

Part nameWiki namePlasmidNumber
<partinfo>BBa_K300005</partinfo>I0pSB1C31
<partinfo>BBa_K300006</partinfo>I1pSB1C32
<partinfo>BBa_K300009</partinfo>I3pSB1C33
<partinfo>BBa_K300008</partinfo>I5pSB1C34
<partinfo>BBa_K300024</partinfo>I7pSB1C35
<partinfo>BBa_K300019</partinfo>I12pSB1C36
<partinfo>BBa_K300030</partinfo>I14pSB1C37
<partinfo>BBa_K300028</partinfo>I15pSB1C38
<partinfo>BBa_K300029</partinfo>I16pSB1C39
<partinfo>BBa_K300025</partinfo>I17pSB1C310
<partinfo>BBa_K300026</partinfo>I18pSB1C311
<partinfo>BBa_K300027</partinfo>I19pSB1C312
<partinfo>BBa_K300002</partinfo>I20pSB1C313
<partinfo>BBa_K300003</partinfo>I21pSB1C314
<partinfo>BBa_K300031</partinfo>I22pSB1C315
<partinfo>BBa_K300081</partinfo>I26pSB1C316
<partinfo>BBa_K300079</partinfo>I31pSB1C317
<partinfo>BBa_K300080</partinfo>I35pSB1C318
<partinfo>BBa_K300086</partinfo>I47pSB1C319
<partinfo>BBa_K300088</partinfo>I48pSB1C320
<partinfo>BBa_K300090</partinfo>I49pSB1C321
<partinfo>BBa_K300082</partinfo>I55pSB1C322
<partinfo>BBa_K300084</partinfo>I78pSB1C323
<partinfo>BBa_K300083</partinfo>I79pSB1C324
<partinfo>BBa_K300010</partinfo>I80pSB1C325
<partinfo>BBa_K300007</partinfo>I70 - BioBrick<partinfo>BBa_K300001</partinfo>26
<partinfo>BBa_K300007</partinfo>I70 - vector<partinfo>BBa_K300001</partinfo>27
<partinfo>BBa_K300004</partinfo>INTEINpSB1C328
<partinfo>BBa_I52002</partinfo>I51<partinfo>BBa_K300000</partinfo>29



In order to obtain better results with screening PCR for the E. coli genome integration project, we perform amplifications using an annealing temperature of 63°C. Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.

The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976.


October, 19th

Wiki update and definition of results.

October, 20th

Wiki update and definition of results.

October, 21st

Wiki update and definition of results.

October, 22nd

Wiki update and definition of results.

October, 23rd

Wiki update and definition of results.