Team:UNIPV-Pavia/Calendar/October/settimana1
From 2010.igem.org
m |
m (→October, 10th) |
||
(23 intermediate revisions not shown) | |||
Line 33: | Line 33: | ||
==October, 4th== | ==October, 4th== | ||
- | ON PCR run | + | ON PCR run and than gel run. |
[[Image:UNIPV10_04_10_10_I74_massive_PCR.jpg|thumb|200px|center|I74 massive PCR screening.]] | [[Image:UNIPV10_04_10_10_I74_massive_PCR.jpg|thumb|200px|center|I74 massive PCR screening.]] | ||
Line 65: | Line 65: | ||
*I77 | *I77 | ||
They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
+ | |||
+ | |||
+ | ---- | ||
+ | |||
+ | |||
+ | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Line 89: | Line 95: | ||
---- | ---- | ||
- | + | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Line 110: | Line 116: | ||
---- | ---- | ||
- | PCR | + | Colony PCR screening for I78 (eleven colonies were picked from LB+Cm agar plate and were inoculated into 5 ml LB+Cm34 and let grow ON for stock). |
PCR was performed this day but it would have been run the following one (too late this evening). | PCR was performed this day but it would have been run the following one (too late this evening). | ||
+ | |||
+ | ---- | ||
+ | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Line 143: | Line 152: | ||
---- | ---- | ||
- | + | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Line 156: | Line 165: | ||
This DNA (and that from I55 and I78 too) was sent to BMR Genomics for sequencing. | This DNA (and that from I55 and I78 too) was sent to BMR Genomics for sequencing. | ||
- | + | ---- | |
+ | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | ||
Line 163: | Line 173: | ||
==October, 9th== | ==October, 9th== | ||
- | < | + | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> |
+ | ---- | ||
+ | A new part was cloned with these ones, named I80. It is the basic part to build self-inducible promoters and was obtained with the following ligation: | ||
+ | I80=I3(in <partinfo>pSB1C3</partinfo>) (S-P) + <partinfo>BBa_F2620</partinfo> (X-P) | ||
+ | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 10th== | ==October, 10th== | ||
+ | Inoculum into 4 ml LB+Amp of: | ||
+ | *I75-1/2/3 | ||
+ | *I76-1/2 | ||
+ | *I77-1/2/3 | ||
+ | for E-P screening; | ||
+ | *I47 | ||
+ | *I48 | ||
+ | *I49 | ||
+ | for transfer into <partinfo>pSB1C3</partinfo>. | ||
+ | |||
+ | They were let grow at +37°C, 220 rpm. | ||
+ | |||
+ | ---- | ||
+ | Transformation of I80 ligation. It was plated on LB+Cm34 agar plate and let grow ON at +37°C. | ||
+ | |||
+ | ---- | ||
+ | <font color='red' size='+2'>We are now moving all our parts to <partinfo>pSB1C3</partinfo> plasmid</font> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> |
Latest revision as of 16:38, 23 October 2010
|
|||||||||||||||||||||||||||||
|
|