Team:UNIPV-Pavia/Calendar/October

From 2010.igem.org

(Difference between revisions)
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<tr><td align="center" valign="top"></td><td align="left"></td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left">
-
98° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73).
+
101° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73).
Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C.  
Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C.  
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</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left">
-
99° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm.  
+
102° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm.  
Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run.
Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run.
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</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left">
-
100° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5.
+
103° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5.
Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm.  
Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm.  
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<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left">
-
101° Bio-Lab -
+
104° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left">
-
102° Bio-Lab -
+
105° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left">
-
103° Bio-Lab -
+
106° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left">
-
104° Bio-Lab -
+
107° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left">
-
105° Bio-Lab -
+
108° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left">
-
106° Bio-Lab -
+
109° Bio-Lab -
</td></tr>
</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left">
-
107° Bio-Lab -
+
110° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left">
-
108° Bio-Lab -
+
111° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left">
-
109° Bio-Lab -
+
112° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left">
-
110° Bio-Lab -
+
113° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left">
-
111° Bio-Lab -
+
114° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left">
-
112° Bio-Lab -
+
115° Bio-Lab -
</td></tr>
</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left">
-
113° Bio-Lab -
+
116° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left">
-
114° Bio-Lab -
+
117° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left">
-
115° Bio-Lab -
+
118° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left">
-
116° Bio-Lab -
+
119° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left">
-
117° Bio-Lab -
+
120° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left">
-
118° Bio-Lab -
+
121° Bio-Lab -
</td></tr>
</td></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr>
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left">
-
119° Bio-Lab -
+
122° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left">
-
120° Bio-Lab -
+
123° Bio-Lab -
</td></tr>
</td></tr>
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left">
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left">
-
121° Bio-Lab - Closing wiki
+
124° Bio-Lab - Closing wiki
</td></tr>
</td></tr>

Revision as of 20:53, 3 October 2010
Home
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Week 1

Week 2

Week 3

Week 4


OCTOBER


Bio-Lab activity proceeds!

Select the day you are interested in to find oout the details!

DATEACTIVITY
October, 1st

101° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73).

Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C.

Miniprep and Nanodrop quantification for: I31, I35, I60 (they were also sent sequencing), INTEIN, pSB1C3-1, pSB1C3-2, pSB1C3-3 and I37 (they were no sent sequencing). Digest and gel run/cut/extraction/quantification of these samples were performed.

ON ligation of: I74: I31 + I35 and INTEIN_C3: INTEIN + pSB1C3.

October, 2nd

102° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm.

Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run.

Transformation of I74 and INTEIN_C3 (1ul) in E. coli TOP10. They were plated on LB+Amp100 and LB+Cm34 agar plates respectively. They were let grow ON at +37°C.

Inoculum of I55-1 into 5 ml LB+Amp. ON growth at +37°C, 220 rpm.

October, 3rd

103° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5.

Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm.

Miniprep and quantification of:I55-1, I55-1bis. I55-1bis was digested E-S for about three hours and than gel run/cut.

Colony PCR for I74-1..6. Gel run for PCR samples and I55 (E-S) and cut for this one.

Gel extraction and quantification of I55.

Ligation of I55 with already available DNA: I75: I55 + I37 and I77: I55 + I57.

Inoculum of I35 (last quantification after gel extraction was too poor; we will digest it again E-P) and I54 into 5 ml LB+Amp: ON growth at +37°C, 220 rpm.

OCTOBER: WEEK 1
October, 4th

104° Bio-Lab -

October, 5th

105° Bio-Lab -

October, 6th

106° Bio-Lab -

October, 7th

107° Bio-Lab -

October, 8th

108° Bio-Lab -

October, 9th

109° Bio-Lab -

OCTOBER: WEEK 2
October, 11th

110° Bio-Lab -

October, 12th

111° Bio-Lab -

October, 13th

112° Bio-Lab -

October, 14th

113° Bio-Lab -

October, 15th

114° Bio-Lab -

October, 16th

115° Bio-Lab -

OCTOBER: WEEK 3
October, 18th

116° Bio-Lab -

October, 19th

117° Bio-Lab -

October, 20th

118° Bio-Lab -

October, 21st

119° Bio-Lab -

October, 22nd

120° Bio-Lab -

October, 23rd

121° Bio-Lab -

OCTOBER: WEEK 4
October, 25th

122° Bio-Lab -

October, 26th

123° Bio-Lab -

October, 27th

124° Bio-Lab - Closing wiki

CALENDAR
March
April
May
June
July
August
September
October
November

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Calendar/October"