"
Team:UNIPV-Pavia/Calendar/October
From 2010.igem.org
| Line 37: | Line 37: | ||
<tr><td align="center" valign="top"></td><td align="left"></td></tr> | <tr><td align="center" valign="top"></td><td align="left"></td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 1st|October, 1st]]</td><td align="left"> | ||
| - | 98° Bio-Lab - | + | 98° Bio-Lab - Trasformed plates were chacked(I70, I71, I72 and I73). |
| + | |||
| + | Transformation of I55 into E. coli DH5-alpha. It was plated on LB+Amp 100 agar plate and let grow ON at +37°C. | ||
| + | |||
| + | Miniprep and Nanodrop quantification for: I31, I35, I60 (they were also sent sequencing), INTEIN, pSB1C3-1, pSB1C3-2, pSB1C3-3 and I37 (they were no sent sequencing). | ||
| + | Digest and gel run/cut/extraction/quantification of these samples were performed. | ||
| + | |||
| + | ON ligation of: I74: I31 + I35 and INTEIN_C3: INTEIN + pSB1C3. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 2nd|October, 2nd]]</td><td align="left"> | ||
| - | 99° Bio-Lab - | + | 99° Bio-Lab - PCR from colony for I55 (eleven colonies were picked).We decided to make glycerol stock for I55-1. Inoculum into 5 ml LB+Amp of the positive culture that was let grow ON at 37°C, 220 rpm. |
| + | |||
| + | Miniprep and quantification for I73-1/2/3/4/5/6. 1,5 hours E-P digest (for screening) and gel run. | ||
| + | |||
| + | Transformation of I74 and INTEIN_C3 (1ul) in E. coli TOP10. They were plated on LB+Amp100 and LB+Cm34 agar plates respectively. They were let grow ON at +37°C. | ||
| + | |||
| + | Inoculum of I55-1 into 5 ml LB+Amp. ON growth at +37°C, 220 rpm. | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/September/settimana5 #October, 3rd|October, 3rd]]</td><td align="left"> | ||
| - | 100° Bio-Lab - | + | 100° Bio-Lab - Glycerol stock for:I55-1, IC-1/2/3/4-4C5. |
| + | |||
| + | Pick of a single non-red colony of INTEIN_C3 and inoculum into 5 ml LB+Cm34. Sample was let grow ON at +37°C, 220 rpm. | ||
| + | |||
| + | Miniprep and quantification of:I55-1, I55-1bis. I55-1bis was digested E-S for about three hours and than gel run/cut. | ||
| + | |||
| + | Colony PCR for I74-1..6. Gel run for PCR samples and I55 (E-S) and cut for this one. | ||
| + | |||
| + | Gel extraction and quantification of I55. | ||
| + | |||
| + | Ligation of I55 with already available DNA: I75: I55 + I37 and I77: I55 + I57. | ||
| + | |||
| + | Inoculum of I35 (last quantification after gel extraction was too poor; we will digest it again E-P) and I54 into 5 ml LB+Amp: ON growth at +37°C, 220 rpm. | ||
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | ||
Revision as of 20:39, 3 October 2010
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
