Team:Tsinghua/experiments

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Experiment Records

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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A:Landing pad (LP) construction and Insertion Project with Att Recomination method and Trandition Landing pad








Results

Group 2(b)
1. PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.
Fan1.JPG Fan2.JPG


2. pUC19 double digestion for eGFP and Chlr, ligate with digested eGFP and Chlr separately. PCR to detect pUC19+eGFP and pUC19+ChlrPCR. All 12 samples are positive.

THU-Fan3.JPG


3. Double digest PE1 and mCherry with SalI and BamHI, double digest PC1 and Kan with KpnI and BamHI, ligate and PCR to detect. No positive results exist among PC1+K.

THU-Fan4.JPG


4. Double digest PEM2 and Kan with KpnI and BamHI as well as PC1. Ligate and using PCR to detect with universal primers. PEM2+K 1 and 5 are positive.

THU-Fan5.JPG


5. Double digest PEMK1 and Chlr with KpnI and EcoRI. Ligate and using PCR to detect with universal primers. PEMK1+Chlr 3-5 are positive.

THU-Fan6.JPG