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Team:Tsinghua/experiments
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| - | linklist = new Array("#exp", "#prt", "experiments/results"); | + | linklist = new Array("#exp", "#prt", "experiments/results", "experiments/dry"); |
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| - | < | + | <p>Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.</p> |
| - | + | <p> | |
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Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)<br/> | Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)<br/> | ||
| - | + | </p><p> | |
Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)<br/> | Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)<br/> | ||
| - | + | </p><p> | |
Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination | Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination | ||
| - | < | + | </p><p> |
Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation) | Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation) | ||
| - | < | + | </p><p> |
Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction | Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction | ||
| - | < | + | </p><p> |
Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III | Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III | ||
| - | < | + | </p><p> |
Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods | Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods | ||
| - | < | + | </p><p> |
Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP | Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP | ||
| - | < | + | </p><p> |
Group 4：Screening Strategy | Group 4：Screening Strategy | ||
| - | < | + | </p><p> |
</div></div> | </div></div> | ||
<a name="prt"></a> | <a name="prt"></a> | ||
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| - | < | + | <p> |
Here are our Experimental Protocols in PDF version. You're advised to view in <a href="http://www.adobe.com/acom/" target=blank>Acrobat</a> or <a href="http://get.adobe.com/reader/" target=blank>Adobe Reader</a> which you can download free from the<a href="http://www.adobe.com" target=blank> Home Website of Adobe® Company</a>, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~ | Here are our Experimental Protocols in PDF version. You're advised to view in <a href="http://www.adobe.com/acom/" target=blank>Acrobat</a> or <a href="http://get.adobe.com/reader/" target=blank>Adobe Reader</a> which you can download free from the<a href="http://www.adobe.com" target=blank> Home Website of Adobe® Company</a>, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~ | ||
| - | </ | + | </p> |
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| - | < | + | <h3>Molecular Cloning</h3> |
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<p><a href="https://static.igem.org/mediawiki/2010/0/0a/THUProtocol_1-1_Isolatio_of_plasmid_DNA.pdf" target=blank>THUProtocol_1-1_Isolatio_of_plasmid_DNA</a><br> | <p><a href="https://static.igem.org/mediawiki/2010/0/0a/THUProtocol_1-1_Isolatio_of_plasmid_DNA.pdf" target=blank>THUProtocol_1-1_Isolatio_of_plasmid_DNA</a><br> | ||
</p><p> | </p><p> | ||
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<a href="https://static.igem.org/mediawiki/2010/4/40/THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA</a> | <a href="https://static.igem.org/mediawiki/2010/4/40/THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA</a> | ||
</p><p> | </p><p> | ||
| - | < | + | <h3>Protein Isolation and Identification</h3> |
| + | <p> | ||
<a href="https://static.igem.org/mediawiki/2010/f/f0/THUProtocol_2-1_Protein_Isolation_for_Prokaryotes.pdf" target=blank>THUProtocol_2-1_Protein_Isolation_for_Prokaryotes</a> | <a href="https://static.igem.org/mediawiki/2010/f/f0/THUProtocol_2-1_Protein_Isolation_for_Prokaryotes.pdf" target=blank>THUProtocol_2-1_Protein_Isolation_for_Prokaryotes</a> | ||
</p><p> | </p><p> | ||
Latest revision as of 17:40, 27 October 2010
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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)
Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)
Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination
Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)
Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction
Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III
Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods
Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP
Group 4：Screening Strategy
Protocols
Here are our Experimental Protocols in PDF version. You're advised to view in Acrobat or Adobe Reader which you can download free from the Home Website of Adobe® Company, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~
Molecular Cloning
THUProtocol_1-1_Isolatio_of_plasmid_DNA
THUProtocol_1-2_DNA_Gel_Extraction
THUProtocol_1-3_Restriction_Enzyme_Digestion
THUProtocol_1-4_Ligation_of_DNA_Fragments
THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation
THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA
THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA
Protein Isolation and Identification
THUProtocol_2-1_Protein_Isolation_for_Prokaryotes
