Team:Tsinghua/experiments

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<h1>Protocols</h1>
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Here are our Experimental Protocols in PDF version. You're advised to view in Acrobat or Adobe Reader which you can download free from the Home Website of Adobe® Company, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~
 
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Here are our Experimental Protocols in PDF version. You're advised to view in Acrobat or Adobe Reader which you can download free from the Home Website of Adobe® Company, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~
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<font size="4"><B>Molecular Cloning</B></font>
<font size="4"><B>Molecular Cloning</B></font>
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Revision as of 15:25, 26 October 2010

Until Jamboree

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Experiment Records

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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A:Landing pad (LP) construction and Insertion Project with Att Recomination method and Trandition Landing pad








Protocols

Here are our Experimental Protocols in PDF version. You're advised to view in Acrobat or Adobe Reader which you can download free from the Home Website of Adobe® Company, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~

Molecular Cloning
THUProtocol_1-1_Isolatio_of_plasmid_DNA

THUProtocol_1-2_DNA_Gel_Extraction

THUProtocol_1-3_Restriction_Enzyme_Digestion

THUProtocol_1-4_Ligation_of_DNA_Fragments

THUProtocol_1-5_PCR

THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation

THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA

THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA

Protein Isolation and Identification
THUProtocol_2-1_Protein_Isolation_for_Prokaryotes

THUProtocol_2-2_Protein_Identification_SDS-PAGE

THUProtocol_1-5_PCR

Results

Group 2(b)
1. PCR: Amplication eGFP, mCherry, Kanamycin resistant gene and Chlr four genes separatly from PIB-eGFP, PBS34, PKD13 and PKD3 plasmids.

Fan1.JPG Fan2.JPG


2. pUC19 double digestion for eGFP and Chlr, ligate with digested eGFP and Chlr separately. PCR to detect pUC19+eGFP and pUC19+ChlrPCR. All 12 samples are positive.

THU-Fan3.JPG


3. Double digest PE1 and mCherry with SalI and BamHI, double digest PC1 and Kan with KpnI and BamHI, ligate and PCR to detect. No positive results exist among PC1+K.

THU-Fan4.JPG


4. Double digest PEM2 and Kan with KpnI and BamHI as well as PC1. Ligate and using PCR to detect with universal primers. PEM2+K 1 and 5 are positive.

THU-Fan5.JPG


5. Double digest PEMK1 and Chlr with KpnI and EcoRI. Ligate and using PCR to detect with universal primers. PEMK1+Chlr 3-5 are positive.

THU-Fan6.JPG