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From 2010.igem.org

Revision as of 15:02, 9 September 2010 (view source) Wishyx (Talk | contribs) (New page: == YX's part == Inoculated Chloramphenicol-LB with bacteria harboring Chloramphenicol resistance gene containing T-vectors. Inoculated the same media with bacteria harboring T-vectors with...) ← Older edit		Latest revision as of 15:11, 9 September 2010 (view source) Wishyx (Talk | contribs) (→YX's part)
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	== YX's part ==		== YX's part ==
-	Inoculated Chloramphenicol-LB with bacteria harboring Chloramphenicol resistance gene containing T-vectors. Inoculated the same media with bacteria harboring T-vectors with scrambled insertion sequence. Shook the media in a 37 centigrade shaker.	+	Repeated the experiments the day before. Doubled the antibiotic dose. The result was fine.
-	Checked the results after 13 hours. Both media became turbid. The one with scrambled sequence became darker in color, indicating possible contamination. Decided to repeat the experiments later.	+	Enzymatic digested the segments from the vector with DraIII for 2 hours.
		+
		+	Ran an agarose gel and recycled the cutout sequence.
		+
		+	Did the ligation reaction with Takara Solution I at 16 centigrade.

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Latest revision as of 15:11, 9 September 2010

YX's part

Repeated the experiments the day before. Doubled the antibiotic dose. The result was fine.

Enzymatic digested the segments from the vector with DraIII for 2 hours.

Ran an agarose gel and recycled the cutout sequence.

Did the ligation reaction with Takara Solution I at 16 centigrade.