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From 2010.igem.org

Module I, group 2(b)

Amplify the plasmids pBMN-PIB, pKD13 and pKD3 with competent cells trans5α. All use 0.1% Amp to select.

Module I, YX and ZY's part:

We digested former PCR products with DraIII for over 17 hours. After agarose electrophoresis, we extracted pure products from the gel and ligated with Takara Ligation Solution I.

Besides, colonies with T vector grew on the plates. We picked several colonies and cultured them in 3ml LB media in the shaker.