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Team:Tsinghua/Notebook/27 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == Using the newly synthesized primers go through a pre-test experiment. PCR system (FastPFU): H2O 31.4μl 5×buffer 10μl dNTP 5μ...) |
(→Module I, DT and Fan's part:) |
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| Line 14: | Line 14: | ||
Total 50μl | Total 50μl | ||
| + | |||
| + | |||
| + | == Module I, Danyang's part: == | ||
| + | Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest. | ||
| + | |||
| + | PCR system (FastPFU): | ||
| + | |||
| + | H2O 36.7μl | ||
| + | 10×buffer 5μl | ||
| + | dNTP 5μl | ||
| + | primer1 1μl | ||
| + | primer2 1μl | ||
| + | template 1μl | ||
| + | FastPFU 0.3μl | ||
| + | Total 50μl | ||
== result == | == result == | ||
The concentration of the M, K and C are a little lower. We’ll amplify them once again with two degrees lower of the annealing temperature. | The concentration of the M, K and C are a little lower. We’ll amplify them once again with two degrees lower of the annealing temperature. | ||
Revision as of 12:59, 3 September 2010
Module I, DT and Fan's part:
Using the newly synthesized primers go through a pre-test experiment.
PCR system (FastPFU):
H2O 31.4μl 5×buffer 10μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.6μl Total 50μl
Module I, Danyang's part:
Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest.
PCR system (FastPFU):
H2O 36.7μl 10×buffer 5μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.3μl Total 50μl
result
The concentration of the M, K and C are a little lower. We’ll amplify them once again with two degrees lower of the annealing temperature.
