Team:Tsinghua/Notebook/24 September 2010


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Module Ⅱ, group 4

Transform the trans5α with OmpA and CipC, respectively.

Transform the trans5α with pET21b.

Inoculation of OmpA、CipC、pET21b, 37℃,12h

Plasmid abstraction (OmpA、CipC、pET21b)

Measuring the concentration of the three kinds of plasmids.


The concentration of OmpA is 125.1ng/ul;

The concentration of CBD is 117.3ng/ul;

The concentration of OmpA is 269.4ng/ul.