Team:Tsinghua/Notebook/23 August 2010

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Module I, group 2c


Digest eGFP with XbaI and EcoRI. digestion system

eGFP           3ul
FD Buffer      2ul
XbaI           0.5ul
Dra III        0.5ul
H2O            14ul

Digest mCherry fragment with Dra III. digestion system

mCherry        3ul
FD Buffer      2ul
Dra III        1ul
H2O            14ul

Digest Kan fragment with Dra III and SpeI. digestion system

Kan            3ul
FD Buffer      2ul
Dra III        0.5ul
SpeI           0.5ul
H2O            14ul

Digest Chlr fragment with EcoRI and PstI. digestion system

Kan            3ul
FD Buffer      2ul
Dra III        0.5ul
SpeI           0.5ul
H2O            14ul

Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to phosphorylate the 5' end of digestion result. digestion system

PSB1C13        4ul
FD Buffer      2ul
XbaI           0.5ul
SpeI           0.5ul
AP             0.5ul
H2O            12.5ul

12h after digestion, the products are purified with kit.