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Team:Tsinghua/Notebook/23 August 2010
From 2010.igem.org
(Difference between revisions)
(→Module I, group 2c) |
(→Module I, group 2c) |
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| Line 29: | Line 29: | ||
Kan 3ul | Kan 3ul | ||
FD Buffer 2ul | FD Buffer 2ul | ||
| - | + | XbaI 0.5ul | |
| - | + | Pst I 0.5ul | |
H2O 14ul | H2O 14ul | ||
---- | ---- | ||
| - | Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to | + | Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion result. |
digestion system | digestion system | ||
PSB1C13 4ul | PSB1C13 4ul | ||
Latest revision as of 20:20, 26 October 2010
Module I, group 2c
Digest eGFP with XbaI and EcoRI. digestion system
eGFP 3ul FD Buffer 2ul XbaI 0.5ul Dra III 0.5ul H2O 14ul
Digest mCherry fragment with Dra III. digestion system
mCherry 3ul FD Buffer 2ul Dra III 1ul H2O 14ul
Digest Kan fragment with Dra III and SpeI. digestion system
Kan 3ul FD Buffer 2ul Dra III 0.5ul SpeI 0.5ul H2O 14ul
Digest Chlr fragment with EcoRI and PstI. digestion system
Kan 3ul FD Buffer 2ul XbaI 0.5ul Pst I 0.5ul H2O 14ul
Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion result. digestion system
PSB1C13 4ul FD Buffer 2ul XbaI 0.5ul SpeI 0.5ul AP 0.5ul H2O 12.5ul
12h after digestion, the products are purified with kit.
