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Team:Tsinghua/Notebook/23 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, group 2c == Run the same PCR program as yesterday and purify the product with gel purification kit. ---- Digest eGFP with XbaI and EcoRI, Ap is uesd to phosphorylate the 5' e...) |
(→Module I, group 2c) |
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== Module I, group 2c == | == Module I, group 2c == | ||
| - | |||
---- | ---- | ||
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Digest mCherry fragment with Dra III,Ap is uesd to phosphorylate the 5' end of digestion result. | Digest mCherry fragment with Dra III,Ap is uesd to phosphorylate the 5' end of digestion result. | ||
digestion system | digestion system | ||
| - | mCherry | + | mCherry 3ul |
FD Buffer 2ul | FD Buffer 2ul | ||
Dra III 1ul | Dra III 1ul | ||
| Line 20: | Line 19: | ||
Digest Kan fragment with Dra III and SpeI,Ap is uesd to phosphorylate the 5' end of digestion result. | Digest Kan fragment with Dra III and SpeI,Ap is uesd to phosphorylate the 5' end of digestion result. | ||
digestion system | digestion system | ||
| - | Kan | + | Kan 3ul |
FD Buffer 2ul | FD Buffer 2ul | ||
Dra III 0.5ul | Dra III 0.5ul | ||
| - | + | SpeI 0.5ul | |
| - | H2O | + | H2O 14ul |
---- | ---- | ||
Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to phosphorylate the 5' end of digestion result. | Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to phosphorylate the 5' end of digestion result. | ||
Revision as of 19:43, 26 October 2010
Module I, group 2c
Digest eGFP with XbaI and EcoRI, Ap is uesd to phosphorylate the 5' end of digestion result. digestion system
eGFP 3ul FD Buffer 2ul XbaI 0.5ul Dra III 0.5ul H2O 14ul
Digest mCherry fragment with Dra III,Ap is uesd to phosphorylate the 5' end of digestion result. digestion system
mCherry 3ul FD Buffer 2ul Dra III 1ul H2O 14ul
Digest Kan fragment with Dra III and SpeI,Ap is uesd to phosphorylate the 5' end of digestion result. digestion system
Kan 3ul FD Buffer 2ul Dra III 0.5ul SpeI 0.5ul H2O 14ul
Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to phosphorylate the 5' end of digestion result. digestion system
PSB1C13 4ul FD Buffer 2ul XbaI 0.5ul SpeI 0.5ul AP 0.5ul H2O 12.5ul
12h after digestion, the products are purified with kit.
