"
Team:Tsinghua/Notebook/20 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, group 2c == Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one. digestion system ...) |
|||
| Line 1: | Line 1: | ||
| - | |||
== Module I, group 2c == | == Module I, group 2c == | ||
Extract plasmid from the clone we Picked yesterday. | Extract plasmid from the clone we Picked yesterday. | ||
| Line 11: | Line 10: | ||
Result: None of the clone picked is positive. | Result: None of the clone picked is positive. | ||
| + | |||
| + | Pick six more clones from the LB plate with 1% Amp. | ||
Latest revision as of 19:12, 26 October 2010
Module I, group 2c
Extract plasmid from the clone we Picked yesterday. Digest the plasmid with EcoRI and run gel to identify whether the clone is a positive one.
digestion system
plasmid 6ul EcoRI 1ul FD Buffer 2ul H2O 11ul
Result: None of the clone picked is positive.
Pick six more clones from the LB plate with 1% Amp.
