Team:Tsinghua/Notebook/16 August 2010

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Module I, group 2(b)

Universal primers of pUC19 are available now (marked p-up and p-do)! Use the universal primers to detect PEM, PKC and PEMKC with SuperMix system shown before.

result

The result shows that PEM plasmids are truly positive but kan gene were not ligated successfully. So that the EMKC are not positive with K missed.

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