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Team:Tsinghua/Notebook/14 July 2010
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| - | == Module I, | + | == Module I, group 2(b)== |
Measure the concentration of the PCR products. | Measure the concentration of the PCR products. | ||
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Productions purify and measure the concentration. | Productions purify and measure the concentration. | ||
| - | Gene Concentration(μg/ml) OD 260/280 | + | Gene Concentration(μg/ml) OD 260/280 |
| - | eGFP 38.4 1.66 | + | eGFP 38.4 1.66 |
| - | mCherry 28.8 1.86 | + | mCherry 28.8 1.86 |
| - | Kan 43.7 2.11 | + | Kan 43.7 2.11 |
| - | Chlr 31.9 1.77 | + | Chlr 31.9 1.77 |
Latest revision as of 06:50, 25 October 2010
Module I, group 2(b)
Measure the concentration of the PCR products.
Digest the four fragments with follow system at 37℃ for 10 hours.
Digestion system:
eGFP:
H2O 23.5μl 10×buffer 5μl eGFP 20μl HindIII 1.5μl Total 50μl
mCherry:
H2O 11.5μl 10×buffer 5μl mCherry 32μl HindIII 1.5μl Total 50μl
Kan:
H2O 31μl 10×buffer 5μl Kan 12.5μl KpnI 1.5μl Total 50μl
Chlr:
H2O 31.5μl 10×buffer 5μl Chlr 12μl KpnI 1.5μl Total 50μl
Productions purify and measure the concentration.
Gene Concentration(μg/ml) OD 260/280 eGFP 38.4 1.66 mCherry 28.8 1.86 Kan 43.7 2.11 Chlr 31.9 1.77
