http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&feed=atom&action=historyTeam:Tokyo Tech/Project/Apple Reporter - Revision history2024-03-28T21:26:47ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=208420&oldid=prevTaichi: /* zeaxanthin synthesis */2010-10-28T03:57:30Z<p><span class="autocomment">zeaxanthin synthesis</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By introduction of ''crtZ'' gene to the ''crtEBIY'' plasmid (we call this construct “single plasmid construct”, "''crtZ-crtEBIY''".), new spot appered on TLC plate (Fig.2-1-6 spot III). The spot shows lower Rf value than that derived from ''crtEBIY'' (Fig.2-1-6 spot II). The Rf values of spots <del class="diffchange diffchange-inline">delived </del>from ''crtEBIY'' and ''crtZ-crtEBIY'' corresponded to reported values of &beta;-caroten and Zeaxanthin, respectively.[https://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter#Reference] Thus we could characterize zeaxanthin production activity of crtZ. In addition, caracterization of the part ''crtZ'' is the first work in iGEM. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By introduction of ''crtZ'' gene to the ''crtEBIY'' plasmid (we call this construct “single plasmid construct”, "''crtZ-crtEBIY''".), new spot appered on TLC plate (Fig.2-1-6 spot III). The spot shows lower Rf value than that derived from ''crtEBIY'' (Fig.2-1-6 spot II). The Rf values of spots <ins class="diffchange diffchange-inline">derived </ins>from ''crtEBIY'' and ''crtZ-crtEBIY'' corresponded to reported values of &beta;-caroten and Zeaxanthin, respectively.[https://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter#Reference] Thus we could characterize zeaxanthin production activity of crtZ. In addition, caracterization of the part ''crtZ'' is the first work in iGEM. </div></td></tr>
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</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=208232&oldid=prevAli.motazedian: /* zeaxanthin synthesis */2010-10-28T03:53:49Z<p><span class="autocomment">zeaxanthin synthesis</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===zeaxanthin synthesis===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===zeaxanthin synthesis===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:tokyotech_Fig. 1.1.6. zeaxanthin.jpg|right|200px|thumb|Fig.2-1-5zeaxanthin]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:tokyotech_Fig. 1.1.6. zeaxanthin.jpg|right|200px|thumb|Fig.2-1-5zeaxanthin]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team tried to synthesize zeaxanthin by the <del class="diffchange diffchange-inline">construct </del>([http://partsregistry.org/wiki/index.php?title=Part:BBa_K395704 BBa_K395704]) to confirm the activity of BioBrick parts designed by Cambredge 2009 and Edinburgh 2007. We also confirmed zeaxanthin by TLC (Fig.2-1-6 spot III). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team tried to synthesize zeaxanthin by <ins class="diffchange diffchange-inline">using </ins>the <ins class="diffchange diffchange-inline">constructed </ins>([http://partsregistry.org/wiki/index.php?title=Part:BBa_K395704 BBa_K395704])<ins class="diffchange diffchange-inline">part </ins>to confirm the activity of BioBrick parts designed by Cambredge 2009 and Edinburgh 2007. We also confirmed zeaxanthin by TLC (Fig.2-1-6 spot III). </div></td></tr>
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</table>Ali.motazedianhttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=208161&oldid=prevTaichi: /* Abstract */2010-10-28T03:51:49Z<p><span class="autocomment">Abstract</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- ここから始まるよ--> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- ここから始まるよ--> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Abstract==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Abstract==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team synthesized &beta;-carotene, zeaxanthin and astaxanthin under several conditions (strain, plasmid copy number,…etc). Particularly, '''astaxanthin is the final metabolite of the carotenoid synthetic pathway.''' </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team synthesized &beta;-carotene, zeaxanthin and astaxanthin under several conditions (strain, plasmid copy number,…etc) <ins class="diffchange diffchange-inline">to confirm the functions of BioBrick parts designed by previous iGEM teams</ins>. Particularly, '''astaxanthin is the final metabolite of the carotenoid synthetic pathway.''' </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized &beta;-carotene and zeaxanthin by assembling ''crtEBIY'' and ''crtZ'' of existing BioBrick parts. '''Though the part ''crtZ'' has been unconfirmed to produce zeaxanthin, we confirmed it for the first time in iGEM.''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized &beta;-carotene and zeaxanthin by assembling ''crtEBIY'' and ''crtZ'' of existing BioBrick parts. '''Though the part ''crtZ'' has been unconfirmed to produce zeaxanthin, we confirmed it for the first time in iGEM.''' </div></td></tr>
</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=208006&oldid=prevTaichi: /* Abstract */2010-10-28T03:45:44Z<p><span class="autocomment">Abstract</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Abstract==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Abstract==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team synthesized &beta;-carotene, zeaxanthin and astaxanthin under several conditions (strain, plasmid copy number,…etc) <del class="diffchange diffchange-inline">to confirm the functions</del>. Particularly, '''astaxanthin is the final metabolite of the carotenoid synthetic pathway.''' </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team synthesized &beta;-carotene, zeaxanthin and astaxanthin under several conditions (strain, plasmid copy number,…etc). Particularly, '''astaxanthin is the final metabolite of the carotenoid synthetic pathway.''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized &beta;-carotene and zeaxanthin by assembling ''crtEBIY'' and ''crtZ'' of existing BioBrick parts. '''Though the part ''crtZ'' has been unconfirmed to produce zeaxanthin, we confirmed it for the first time in iGEM.''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized &beta;-carotene and zeaxanthin by assembling ''crtEBIY'' and ''crtZ'' of existing BioBrick parts. '''Though the part ''crtZ'' has been unconfirmed to produce zeaxanthin, we confirmed it for the first time in iGEM.''' </div></td></tr>
</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207343&oldid=prevKino: /* zeaxanthin synthesis */2010-10-28T03:26:16Z<p><span class="autocomment">zeaxanthin synthesis</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Constructs containing pBad/araC promoter on high-copy vector show the best production and double plasmids construct shows as much production as single plasmid construct under same promoter combination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.(Actually, <del class="diffchange diffchange-inline">#</del>3 showed a little more production than <del class="diffchange diffchange-inline">#</del>5.) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Constructs containing pBad/araC promoter on high-copy vector show the best production and double plasmids construct shows as much production as single plasmid construct under same promoter combination. Construct with pBad/araC promoter shows better production than that with plac promoter. Construct that ''crtEBIY'' on high-copy vector and ''crtZ'' on low-copy vector shows as much expression as construct that ''crtEBIY'' on low-copy vector and ''crtZ'' on high-copy vector.(Actually, 3 showed a little more production than 5.) </div></td></tr>
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</table>Kinohttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207261&oldid=prevTaichi: /* astaxanthin synthesis */2010-10-28T03:23:54Z<p><span class="autocomment">astaxanthin synthesis</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:<del class="diffchange diffchange-inline">tokyotech_Fig. 2</del>-<del class="diffchange diffchange-inline">1-10 1BBa_K395706</del>.jpg|left|300px|thumb|S2 : astaxanthin expression component]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:<ins class="diffchange diffchange-inline">S2</ins>-<ins class="diffchange diffchange-inline">astaxanthin</ins>.jpg|left|300px|thumb|S2 : astaxanthin expression component]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:TLC work3.jpeg|right|300px|thumb|Fig.2-1-6 TLC astaxanthin worked by Yumiko Kinoshita]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:TLC work3.jpeg|right|300px|thumb|Fig.2-1-6 TLC astaxanthin worked by Yumiko Kinoshita]]</div></td></tr>
</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207235&oldid=prevTaichi: /* zeaxanthin synthesis */2010-10-28T03:23:20Z<p><span class="autocomment">zeaxanthin synthesis</span></p>
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</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207202&oldid=prevTaichi: /* astaxanthin synthesis */2010-10-28T03:22:34Z<p><span class="autocomment">astaxanthin synthesis</span></p>
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</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207177&oldid=prevTaichi: /* zeaxanthin synthesis */2010-10-28T03:21:58Z<p><span class="autocomment">zeaxanthin synthesis</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:TLC work3.jpeg|right|300px|thumb|Fig.2-1-6 zeaxanthin TLC worked by Yumiko Kinoshita]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:TLC work3.jpeg|right|300px|thumb|Fig.2-1-6 zeaxanthin TLC worked by Yumiko Kinoshita]]</div></td></tr>
</table>Taichihttp://2010.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Project/Apple_Reporter&diff=207173&oldid=prevKino: /* astaxanthin synthesis */2010-10-28T03:21:53Z<p><span class="autocomment">astaxanthin synthesis</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 03:21, 28 October 2010</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By introduction of ''crtW'' gene to the ''crtZ'' plasmid (the second construct of five combinations in “zeaxanthin synthesis”,having pBad/araC promoter), new spot ((Fig2-1-6 spot IV) appeared on TLC plate. The spot shows lower Rf value than that derived from ''crtEBIY'' (II) and a little higher Rf value than that derived from ''crtZ-crtW''(III). All the Rf values of spots corresponded to reported values [https://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter#Reference [2]] <del class="diffchange diffchange-inline">respectively. </del>Thus we succeeded in characterizing astaxanthin production activity of ''crtW''. ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K395705 BBa_K395705], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K395706 BBa_K395706]) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By introduction of ''crtW'' gene to the ''crtZ'' plasmid (the second construct of five combinations in “zeaxanthin synthesis”,having pBad/araC promoter), new spot ((Fig2-1-6 spot IV) appeared on TLC plate. The spot shows lower Rf value than that derived from ''crtEBIY'' (II) and a little higher Rf value than that derived from ''crtZ-crtW''(III). All the Rf values of spots corresponded to reported values <ins class="diffchange diffchange-inline"> respectively.</ins>[https://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter#Reference [2]] Thus we succeeded in characterizing astaxanthin production activity of ''crtW''. ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K395705 BBa_K395705], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K395706 BBa_K395706]) </div></td></tr>
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</table>Kino