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Team:TU Delft/Project/solubility/results
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| + | {{Team:TU_Delft/frame_check}} | ||
| + | __NOTOC__ | ||
==Hydrocarbon Solubility Results & Conclusions== | ==Hydrocarbon Solubility Results & Conclusions== | ||
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===Results=== | ===Results=== | ||
| - | + | ||
| + | For the production of large amounts of protein, 1 L shake flasks cultures were induced with IPTG after the OD600 was starting to increase towards 0.1 (Table 1). | ||
| + | |||
| + | {| | ||
| + | |'''#''' | ||
| + | |'''Culture''' | ||
| + | |'''OD600 at induction''' | ||
| + | |'''OD600 at harvest''' | ||
| + | |- | ||
| + | |1 | ||
| + | |Control (J13002) 30 C | ||
| + | |0.073 | ||
| + | |0.451 | ||
| + | |- | ||
| + | |2 | ||
| + | |Control (J13002) 37 C | ||
| + | |0.088 | ||
| + | |0.810 | ||
| + | |- | ||
| + | |3 | ||
| + | |AlnA (K398206) 30 C | ||
| + | |0.060 | ||
| + | |0.393 | ||
| + | |- | ||
| + | |4 | ||
| + | |AlnA (K398206) 37 C | ||
| + | |0.067 | ||
| + | |0.624 | ||
| + | |} | ||
===Conclusions=== | ===Conclusions=== | ||
jadie jadie jadie | jadie jadie jadie | ||
Revision as of 10:07, 20 October 2010
Hydrocarbon Solubility Results & Conclusions
Our goal was to enhance the solubility of alkanes in water. Therefore we constructed a new part that expresses the emulsifier protein AlnA after induction by IPTG. By using our own emulsification assay we measured the increase in hydrophobic compounds in the water phase.
Results
For the production of large amounts of protein, 1 L shake flasks cultures were induced with IPTG after the OD600 was starting to increase towards 0.1 (Table 1).
| # | Culture | OD600 at induction | OD600 at harvest |
| 1 | Control (J13002) 30 C | 0.073 | 0.451 |
| 2 | Control (J13002) 37 C | 0.088 | 0.810 |
| 3 | AlnA (K398206) 30 C | 0.060 | 0.393 |
| 4 | AlnA (K398206) 37 C | 0.067 | 0.624 |
Conclusions
jadie jadie jadie
