Team:TU Delft/Project/solubility/characterization

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(New page: =Characterization of the Solubility Parts= ==Emulsifier Production== The alna gene was induced when the culture reached a density of about 108 bacteria per mL. Bacterial and medium sample...)
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Characterization of the Solubility Parts

Emulsifier Production

The alna gene was induced when the culture reached a density of about 108 bacteria per mL. Bacterial and medium samples were taken for sodium dodecyl sulfate (SDS)-gel electrophoresis to monitor AlnA production. The results shown in Fig. ? indicate that expression of AlnA begins ? min after induction and peaks after ? h.

Emulsifier Assay

Calibration graph showing a linear increase in absorbance at 660 nm for increasing concentrations of SDS (●). Under the standard detergent assay conditions, various components like 200 mM NaCl (□), 2.0 mM CaCl2 (♦), 10% glycerol (■), 100 μg microsomal membranes (open diamond), 0.2 mM Triton X-100 (▲) and 2.5 mM CHAPS (○) were added and the turbidity was measured. Error bars represent the deviation from five independent experiments and each one performed in duplicate. Rajakumari et al (2006) Biochemical and Biophysical Methods
We developed a new assay to measure the emulsification caused by AlnA. Assays currently described in literature involve spectrophotometric measurements of turbidity after mixing. Although many articles show nice graphs (see textbox on the right), we were unable to reproduce them. Measuring turbidity turned out to be rather arbitrary.
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