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| | * [[Team:TU_Delft/Project/rbs-characterization/characterization|Characterization]] | | * [[Team:TU_Delft/Project/rbs-characterization/characterization|Characterization]] |
| | * [[Team:TU_Delft/Project/rbs-characterization/results|Results and Conclusions]] | | * [[Team:TU_Delft/Project/rbs-characterization/results|Results and Conclusions]] |
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| - | ==Experimental Setup==
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| - | We took five different RBS sequences from the [http://partsregistry.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Anderson Anderson RBS family] (J61100, J61101, J61107, J61117, J61127). The standard RBS [http://partsregistry.org/Part:BBa_B0032 B0032] was used as a reference for our characterization.
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| - | All these RBS sequences were placed in front of the standard [http://partsregistry.org/Part:BBa_E0040 GFP coding sequence], so expression could be measured.
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| - | The Biobricks generated in order to perform the experiments were: [http://partsregistry.org/Part:BBa_K398500 K398500], [http://partsregistry.org/Part:BBa_K398501 K398501], [http://partsregistry.org/Part:BBa_K398502 K398502], [http://partsregistry.org/Part:BBa_K398503 K398503], [http://partsregistry.org/Part:BBa_K398504 K398504]. The general map of the construction is shown below, where the RBS is displayed in fucsia.
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| - | [[Image:RBS1.jpg|left|650px]]
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| - | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
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| - | |'''Feature'''
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| - | |'''Function'''
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_B0032 AmpR]
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| - | |Ampicillin resistance
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_B0015 B0015]
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| - | |Transcriptional (double) terminator
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_B0062 B0062]
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| - | |Transcriptional terminator
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_E0040 E0040]
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| - | |GFP
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_G00000 G00000]
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| - | |Standard prefix
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_G00001 G00001]
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| - | |Standard suffix
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_G00100 G00100]
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| - | |VF2 primer binding site
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_G00101 G00101]
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| - | |VR primer binding site
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_J61100 J61100]
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| - | |RBS Anderson family
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| - | |-
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| - | |[http://partsregistry.org/Part:BBa_J23100 J23100]
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| - | |Promoter
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| - | |}
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| - |
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| - | <html><div style='clear:both'>
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| - | </html>
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Part of our project is to measure the effect of various ribosome binding site (RBS) sequences. A RBS sequence is a specific mRNA sequence that folds in such a way that it attracts the ribosome. This ribosome binds to the mRNA molecule and starts translation of the mRNA into protein. Accurate information on how well this binding occurs (The binding strength) can be very useful when designing new biological systems.
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