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Team:TU Delft/Parts/characterization
From 2010.igem.org
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===Favorite Parts=== | ===Favorite Parts=== | ||
| + | ====[http://partsregistry.org/Part:BBa_K398029 BBa_K398029]==== | ||
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| + | [[Image:TUDelftALDH_final.jpg|600px]] | ||
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| + | Our results suggest that the recombinant strain ''E. coli'' 029A functionally express our biobrick [http://partsregistry.org/Part:BBa_K398029 BBa_K398029]. From the statistical analysis that we performed we concluded that the expression of the biobrick [http://partsregistry.org/Part:BBa_K398006 BBa_K398006] under the promoter-rbs combination [http://partsregistry.org/Part:BBa_J13002 BBa_J23100]-[http://partsregistry.org/Part:BBa_J13002 BBa_J61117] increases the dodecanal dehydrogenase activity in ''E. coli'' cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct [http://partsregistry.org/Part:BBa_K398029 BBa_K398029] were equivalent to 33.98% of the ''Pseudomonas putida'' aldehyde dehydrogenase activity. | ||
| + | Read more about it in [https://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results our results page] | ||
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====BBa_K398206==== | ====BBa_K398206==== | ||
[[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject. | [[Image:TU_Delft_Emuls_assay_exp1.jpg|thumb|400px|right|Emulsification of Sudan II by the isolated proteins from the control (BBa_J13002) and AlnA (BBa_K398206) culture. Read more about the [[Team:TU_Delft/Project/solubility/results|BBa_K398206 characteristics]]]]This part contains an IPTG inducible protomor with a protein coding sequence for the production of the emulsifier AlnA. It was the main subject of our [[Team:TU_Delft/Project/solubility|solubility]] subproject. | ||
Revision as of 01:38, 27 October 2010
Characterization
Our favorite parts were also the ones best characterized. This page is a quick overview of the results.
Favorite Parts
BBa_K398029
Our results suggest that the recombinant strain E. coli 029A functionally express our biobrick BBa_K398029. From the statistical analysis that we performed we concluded that the expression of the biobrick BBa_K398006 under the promoter-rbs combination BBa_J23100-BBa_J61117 increases the dodecanal dehydrogenase activity in E. coli cell extracts 2-fold. Moreover, the enzymatic activities measured for the construct BBa_K398029 were equivalent to 33.98% of the Pseudomonas putida aldehyde dehydrogenase activity. Read more about it in our results page
BBa_K398206
View BBa_K398206 in the parts registry
Specified Components <partinfo>K398206 SpecifiedComponents</partinfo>
Designer: <partinfo>K398206 Designer</partinfo>
Status: <partinfo>K398206 Status</partinfo>
