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Team:TU Delft/Modeling/MFA
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==Metabolic Flux Analysis== | ==Metabolic Flux Analysis== | ||
| - | A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to ''E. coli'' along with the hydrocarbon degradation from our | + | A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to ''E. coli'' along with the hydrocarbon degradation from our BioBricks to see what the maximal theoretical yields on alkanes are compared to glucose. |
How a metabolic flux analysis works, is explained [[Team:TU_Delft/Modeling/MFA/explanation|here.]] | How a metabolic flux analysis works, is explained [[Team:TU_Delft/Modeling/MFA/explanation|here.]] | ||
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| - | + | Do want to know the details of the implemented pathways. Check our [[Team:TU_Delft/Modeling/MFA/results|Detailed MFA results]] page | |
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| - | [[Team:TU_Delft/Modeling/MFA/results|Detailed MFA results]] | + | |
==Visualization of pathways== | ==Visualization of pathways== | ||
Revision as of 08:40, 19 October 2010
Metabolic Flux Analysis
A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to E. coli along with the hydrocarbon degradation from our BioBricks to see what the maximal theoretical yields on alkanes are compared to glucose.
How a metabolic flux analysis works, is explained here.
For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified E. coli metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. The resulting network is the network used for adding additional pathways.
Implemented pathways
Do want to know the details of the implemented pathways. Check our Detailed MFA results page
Visualization of pathways
