Team:TU Delft/Brainstorming

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Yeast/bacteria that resist change in their environment (such as pH, temperature, pressure; looking at extremophiles)
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Possibilities:
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*Temperature could be difficult, because we would have to change the temperature externally. Cooling down of an organism can have allot of problems.
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*Metabolite production for endothermic reactions extracellular
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*Antifreeze proteins (bind to ice)
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*pH; this has never been done before
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*Metal sequestering
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*Many different biobricks; differentiation into different possible effects.
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*Using characteristics of extremophiles
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Revision as of 15:08, 9 July 2010

Brainstorming

Brainstorming method

We have tried to set up our brainstorming sessions in such a way that creativity was stimulated and no important areas where missed:

  • First we enumerated problems which might be solvable by synthetic biology, then we gathered as many ways to solve them as possible. It's important to allow any idea to be mentioned here, without any cricital judging of team members yet.
  • In later rounds, we voted on which ideas to work out, split up in teams and assessed the ideas in terms of feasibility, cost, coolness and how useful they could be for synthetic biology.
  • We ended with 3 detailed project ideas and voted on them, and oil degradation was chosen!

Bacterial buffers

Yeast/bacteria that resist change in their environment (such as pH, temperature, pressure; looking at extremophiles) Possibilities:

  • Temperature could be difficult, because we would have to change the temperature externally. Cooling down of an organism can have allot of problems.
  • Metabolite production for endothermic reactions extracellular
  • Antifreeze proteins (bind to ice)
  • pH; this has never been done before
  • Metal sequestering
  • Many different biobricks; differentiation into different possible effects.
  • Using characteristics of extremophiles

Hugo's Risk Scale

Our own standard for project risk assessment. Risk is represented with a value from 0 (no risk) to 10 (very risky)

  • 0: Lac operon (or other piece of cake) + our biobrick
  • 1: Less than 5 genes or biobricks (copy-paste) + our biobrick
  • 2: Less than 10 genes or biobricks (copy-paste) + our biobrick
  • 3: Biobrick engineering <5, improvement of things already done
  • 4: Multi biobrick engineering >5, improvement of things already done
  • 5: Site directed mutagenesis of several genes, results unknown a priori
  • 6: Evolutionary engineering involved = sequencing
  • 7: Protein engineering involved, results unknown a priori
  • 8: A lot of genes and/or biobricks (>20), known genes in other species and characterized. Stress, social friction
  • 9: A lot of genes and/or biobricks (>20), Known genes in other species, not fully characterized. Start a project one-eyed = stress, social friction, high chances of collapse
  • 10: A lot of genes (>20), Unknown genes= characterization + sequencing + cloning. Start a project from nothing or partially blind, team-member losses + obituraries. Good side: paper in Nature or Nobel