Team:TU Delft/6 July 2010 content

From 2010.igem.org

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===Lab work===
===Lab work===
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The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!
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Emulsifying Assay
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We were so excited; we immediately solved the DNA in water and performed a [[Team:TU_Delft/protocols/transformation|transformation]] with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.
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1. AlkB2 on 100 μg/mL Ampicillin
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2. rubA3 on 100 μg/mL Ampicillin
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3. rubA4 on 100 μg/mL Ampicillin
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4. rubB on 50 μg/mL Kanamycin
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5. ladA on 100 μg/mL Ampicillin
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6. ADH on 100 μg/mL Ampicillin
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7. ALDH on 50 μg/mL Kanamycin
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8. bbc1 on 100 μg/mL Ampicillin
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9. AlnA on 100 μg/mL Ampicillin
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10. OprG on 100 μg/mL Ampicillin
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11. PalkS1-2 on 100 μg/mL Ampicillin
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12. PalkB on 100 μg/mL Ampicillin
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13. P(CaiF) on 100 μg/mL Ampenicillin
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14. AlkS on 50 μg/mL Kanamycin
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15. PhPFD-α on 100 μg/mL Ampicillin
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16. PhPFD-β on 100 μg/mL Ampicillin
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Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:
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To determine the emulsifying activity we set up a calibration curve with SDS. Pieter prepared the following samples:
 
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Revision as of 19:08, 12 July 2010

Lab work

The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!

We were so excited; we immediately solved the DNA in water and performed a transformation with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.

1. AlkB2 on 100 μg/mL Ampicillin 2. rubA3 on 100 μg/mL Ampicillin 3. rubA4 on 100 μg/mL Ampicillin 4. rubB on 50 μg/mL Kanamycin 5. ladA on 100 μg/mL Ampicillin 6. ADH on 100 μg/mL Ampicillin 7. ALDH on 50 μg/mL Kanamycin 8. bbc1 on 100 μg/mL Ampicillin 9. AlnA on 100 μg/mL Ampicillin 10. OprG on 100 μg/mL Ampicillin 11. PalkS1-2 on 100 μg/mL Ampicillin 12. PalkB on 100 μg/mL Ampicillin 13. P(CaiF) on 100 μg/mL Ampenicillin 14. AlkS on 50 μg/mL Kanamycin 15. PhPFD-α on 100 μg/mL Ampicillin 16. PhPFD-β on 100 μg/mL Ampicillin


Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:

Hexane (mL) Tris Buffer pH 8 (mL) 10% SDS (mL)
B 1 1.1 0
100 1 1 0.1
50 1 1.05 0.05
25 1 1.075 0.025
10 1 1.09 0.01
5 1 1.095 0.005


Kampioenen!!!!

Today the Dutch team will play the semi-final of the World Cup! Hear us cheer! Stop sound

Pieter by the BBQ
We are ready for the soccer game
the Netherlands won the semi-final!