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Team:TU Delft/6 August 2010 content
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[https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=05_August_2010 Yesterday's] plates showed colonies. To check whether these contained the desired insert a colony PCR was performed. The products of this PCR were checked on a gel. | [https://2010.igem.org/Team:TU_Delft#page=pages/blog&blog=05_August_2010 Yesterday's] plates showed colonies. To check whether these contained the desired insert a colony PCR was performed. The products of this PCR were checked on a gel. | ||
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[[Image:TUDelft_20100806_pcr.png|450px]] | [[Image:TUDelft_20100806_pcr.png|450px]] | ||
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Revision as of 16:10, 15 August 2010
Lab work
Alkane degration
Yesterday's plates showed colonies. To check whether these contained the desired insert a colony PCR was performed. The products of this PCR were checked on a gel.
On the gel, two colonies of 011A showed the right length. None of the lengths for 013A or K were correct. They did however match the PCR length (009A, 482) of one the inserts which was digested in order to create this BioBrick. Next week we will take a few more colonies from the plate and check these, and otherwise the digestion and ligation will be tried again.
Lane description
| # | Description | Primers | Expected length (bp) | ✓ | ✗ |
| L | SmartLadder | n/a | n/a | n/a | n/a |
| 1-5 | 011A | G00100 + G00101 | 1816 | 1,5 | 2,3,4 |
| 6-10 | 013A | G00100 + G00101 | 1929 | none | 6,7,8,9,10 |
| 11-15 | 013K | G00100 + G00101 | 1929 | none | 11,12,13,14,15 |
| L | SmartLadder | n/a | n/a | n/a | n/a |
