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Team:TU Delft/29 July 2010 content
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(New page: =Lab work= ==Alkane Degradation== <h4>PCR Amplification</h4> K398007A, K398008A, K398009A, K398010A, K398017A, K398018A, K398019A, B0015 were amplified w...) |
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| Line 25: | Line 25: | ||
|1 | |1 | ||
|PCR product of 007 | |PCR product of 007 | ||
| - | | | + | |1616 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 32: | Line 32: | ||
|2 | |2 | ||
|PCR product of 008 | |PCR product of 008 | ||
| - | | | + | |473 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 39: | Line 39: | ||
|3 | |3 | ||
|PCR product of 009 | |PCR product of 009 | ||
| - | | | + | |482 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 46: | Line 46: | ||
|4 | |4 | ||
|PCR product of 010 | |PCR product of 010 | ||
| - | | | + | |1505 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 53: | Line 53: | ||
|5 | |5 | ||
|PCR product of 017 | |PCR product of 017 | ||
| - | | | + | |1625 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 60: | Line 60: | ||
|6 | |6 | ||
|PCR product of 018 | |PCR product of 018 | ||
| - | | | + | |1052 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 67: | Line 67: | ||
|7 | |7 | ||
|PCR product of 019 | |PCR product of 019 | ||
| - | | | + | |1796 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| Line 74: | Line 74: | ||
|8 | |8 | ||
|PCR product of B0015 | |PCR product of B0015 | ||
| - | | | + | |445 |
|G00101 + G00101 | |G00101 + G00101 | ||
|<font color=limegreen>✔</font> | |<font color=limegreen>✔</font> | ||
| | | | ||
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Revision as of 06:55, 2 August 2010
Lab work
Alkane Degradation
PCR Amplification
K398007A, K398008A, K398009A, K398010A, K398017A, K398018A, K398019A, B0015 were amplified with the universal primers G00100 and G00101. The product was put on 1% agarose gel:
Lane Description:
| # | Description | Expected Length (bp) | Primers | Status | Remarks |
| M1 | SmartLadder | n/a | n/a | n/a | |
| 1 | PCR product of 007 | 1616 | G00101 + G00101 | ✔ | |
| 2 | PCR product of 008 | 473 | G00101 + G00101 | ✔ | |
| 3 | PCR product of 009 | 482 | G00101 + G00101 | ✔ | |
| 4 | PCR product of 010 | 1505 | G00101 + G00101 | ✔ | |
| 5 | PCR product of 017 | 1625 | G00101 + G00101 | ✔ | |
| 6 | PCR product of 018 | 1052 | G00101 + G00101 | ✔ | |
| 7 | PCR product of 019 | 1796 | G00101 + G00101 | ✔ | |
| 8 | PCR product of B0015 | 445 | G00101 + G00101 | ✔ |
