Team:TU Delft/23 June 2010 content


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Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:

- μL
I10341 3
J611XX 4
pSB3C5 1.5
Ligation Buffer 3
T4 ligase 1 unit
H20 15

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.