Team:TU Delft/23 June 2010 content

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LAB WORK

Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:

Componet Sample
I10341 3 μL
J611XX 4 μL
pSB3C5 1.5 μL
Ligation Buffer 3 μL
T4 ligase 1 μL
H20 15 μL


We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5. The mixtures were incubated overnight at 16 degrees.