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From 2010.igem.org

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

#	Description	Expected length (bp)
1	[SmartLadder] (5 μL)	n/a
2-4	007T	1616
5-6	008T	551
7-9	009T	560
10-12	010T	1657
13-15	017T	1130
16	018T	1874
17	Red colony	n/a

A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will do a plasmid isolation with the cultures of lane 2, 4, 6, 7, 9 and 14. We will cut the isolated plasmids with various restriction enzymes and analyze the digestion products on gel.