Team:TU Delft/22 July 2010 content

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Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples

|# |Description |Expected length (bp) |- |1 |[SmartLadder] (5 μL) |n/a |- |2 |007T |1616 |- |3 |007T |1616 |- |4 |007T |1616 |- |5 |008T |551 |- |6 |008T |551 |- |7 |009T |560 |- |8 |009T |560 |- |9 |009T |560 |- |10 |010T |1657 |- |11 |010T |1657 |- |12 |010T |1657 |- |13 |017T |1130 |- |14 |017T |1130 |- |15 |017T |1130 |- |16 |018T |1874 |- |17 |Red colony |n/a |}