Team:TU Delft/22 July 2010 content

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A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will cut them with various restriction enzymes and analyze the digestion products on gel.
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A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will do a plasmid isolation with the cultures of lane 2, 4, 6, 7, 9 and 14. We will cut the isolated plasmids with various restriction enzymes and analyze the digestion products on gel.

Revision as of 10:25, 26 July 2010

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples
# Description Expected length (bp)
1 [SmartLadder] (5 μL) n/a
2-4 007T 1616
5-6 008T 551
7-9 009T 560
10-12 010T 1657
13-15 017T 1130
16 018T 1874
17 Red colony n/a

A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will do a plasmid isolation with the cultures of lane 2, 4, 6, 7, 9 and 14. We will cut the isolated plasmids with various restriction enzymes and analyze the digestion products on gel.