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Revision as of 07:40, 23 July 2010 (view source) Kschipper (Talk | contribs) (→Alkane degradation) ← Older edit		Revision as of 10:24, 26 July 2010 (view source) Kschipper (Talk | contribs) (→Alkane degradation) Newer edit →
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		+	A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will cut them with various restriction enzymes and analyze the digestion products on gel.

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Revision as of 10:24, 26 July 2010

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

#	Description	Expected length (bp)
1	[SmartLadder] (5 μL)	n/a
2-4	007T	1616
5-6	008T	551
7-9	009T	560
10-12	010T	1657
13-15	017T	1130
16	018T	1874
17	Red colony	n/a

A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will cut them with various restriction enzymes and analyze the digestion products on gel.