Team:TU Delft/22 July 2010 content

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Revision as of 07:40, 23 July 2010

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples

#	Description	Expected length (bp)
1	[SmartLadder] (5 μL)	n/a
2-4	007T	1616
5-6	008T	551
7-9	009T	560
10-12	010T	1657
13-15	017T	1130
16	018T	1874
17	Red colony	n/a

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 There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a [[Team:TU_Delft/protocols/colony PCR|colony PCR]].
-[[Image:TUDelft_scPCR_22-07.png|400px|thumb|left|1% Agarose gel of SC PCR samples]]
+[[Image:TUDelft_scPCR_22-07.png|550px|thumb|left|1% Agarose gel of SC PCR samples]]
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