Team:TU Delft/22 July 2010 content

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(New page: ==Alkane degradation== There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen...)
(→Alkane degradation)
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==Alkane degradation==
==Alkane degradation==
There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a [[Team:TU_Delft/protocols/colony PCR|colony PCR]].
There were some colonies on [https://2010.igem.org/Team:TU_Delft#/blog?blog=19_July_2010 Tuesday's] plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a [[Team:TU_Delft/protocols/colony PCR|colony PCR]].
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[[Image:TUDelft_scPCR_22-07.png|400px|thumb|left|1% Agarose gel of SC PCR samples]]
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|'''#'''
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|'''Description'''
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|'''Expected length (bp)'''
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|-
 +
|1
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|[[https://2010.igem.org/Image:TU_Delft_SmartLadder.jpg SmartLadder]] (5 μL)
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|n/a
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|-
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|2
 +
|007T
 +
|1616
 +
|-
 +
|3
 +
|007T
 +
|1616
 +
|-
 +
|4
 +
|007T
 +
|1616
 +
|-
 +
|5
 +
|008T
 +
|551
 +
|-
 +
|6
 +
|008T
 +
|551
 +
|-
 +
|7
 +
|009T
 +
|560
 +
|-
 +
|8
 +
|009T
 +
|560
 +
|-
 +
|9
 +
|009T
 +
|560
 +
|-
 +
|10
 +
|010T
 +
|1657
 +
|-
 +
|11
 +
|010T
 +
|1657
 +
|-
 +
|12
 +
|010T
 +
|1657
 +
|-
 +
|13
 +
|017T
 +
|1130
 +
|-
 +
|14
 +
|017T
 +
|1130
 +
|-
 +
|15
 +
|017T
 +
|1130
 +
|-
 +
|16
 +
|018T
 +
|1874
 +
|-
 +
|17
 +
|Red colony
 +
|n/a
 +
|}

Revision as of 07:37, 23 July 2010

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples

|# |Description |Expected length (bp) |- |1 |[SmartLadder] (5 μL) |n/a |- |2 |007T |1616 |- |3 |007T |1616 |- |4 |007T |1616 |- |5 |008T |551 |- |6 |008T |551 |- |7 |009T |560 |- |8 |009T |560 |- |9 |009T |560 |- |10 |010T |1657 |- |11 |010T |1657 |- |12 |010T |1657 |- |13 |017T |1130 |- |14 |017T |1130 |- |15 |017T |1130 |- |16 |018T |1874 |- |17 |Red colony |n/a |}

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