Team:TU Delft/1 July 2010 content

From 2010.igem.org

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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
|'''Sample'''  
|'''Sample'''  
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|'''medium'''
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|'''Medium'''
|'''day 1 = 30 June'''
|'''day 1 = 30 June'''
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|'''day 2= 1 July'''
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|'''day 2 = 1 July'''
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|no cells
|no cells

Revision as of 17:22, 31 July 2010

Lab Work

Pseudomonas Putida GPO1

The second day of the grow process of Pseudomonas Putida GPO1

Sample Medium day 1 = 30 June day 2 = 1 July
no cells E2 without carbon 0.00 0.00
no cells LB 0.00 0.00
no cells E2 + 1% LB 0.00 0.01
Pseudomonas Putida GPO1 * E2 + 1% LB 0.01 0.05
no cells E2 + 10% LB 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% LB 0.34 0.19
no cells E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% octane 0.29 0.18

* average of 3 colonies

Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.

Competent cells

Top10 cells were cultivated for making competent cells.