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Revision as of 21:40, 6 July 2010 (view source) NinaSchiller (Talk | contribs) (→Testing DH5α competent cells by transformation) ← Older edit		Revision as of 21:40, 6 July 2010 (view source) NinaSchiller (Talk | contribs) (→Preparation LB agar plates with ampicillin resistance) Newer edit →
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	# Cool down		# Cool down
	# Add 200 mg ampiciliin in 1,8 ml water		# Add 200 mg ampiciliin in 1,8 ml water
-	# Pour our on plates	+	# Pour out on plates

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Revision as of 21:40, 6 July 2010

Nina

Testing DH5α competent cells by transformation

This was performed according to tranformation protocol From NEB 5-alpha competent E.coli (High Efficiency) NEW ENGLAND BioLabs.

I transformed both my own cells from yesterday and also the kit's competent cells. Each one had a two serial 10-fold dilution.

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Preparation LB agar plates with ampicillin resistance

LB agar:

• 25 g LB
• 15 g bactoagar
• Fill up to 1 L with distilled water

1. Autoclave for 120°C, 20 min
2. Cool down
3. Add 200 mg ampiciliin in 1,8 ml water
4. Pour out on plates