Team:Stockholm/7 June 2010

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Nina

Preparation of competent DH5α Escherichia coli cells

Autoclaving bottles & solutions for next day:

  • Two 1000 ml empty bottles
  • 500 ml 50 mM CaCl2 for 1000 ml cell culture
  • 16 ml 50 mM CaCl2 + 15 % Glycerol

Set a 5 ml LB ON culture of DH5α in 37°C, 250 rpm.