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From 2010.igem.org

Contents 1 Andreas 1.1 Transfer of pEX.nCPP⋅SOD⋅His to BL21 1.1.1 Glycerol stocks 1.2 Assembly of His⋅SOD⋅cCPP constructs 1.2.1 Colony PCR 1.2.2 Gel verification

Andreas

Transfer of pEX.nCPP⋅SOD⋅His to BL21

Glycerol stocks

• BL21 pEX.nLMWP⋅SOD⋅His
• BL21 pEX.nTra10⋅SOD⋅His

Assembly of His⋅SOD⋅cCPP constructs

Colony PCR

1. pSB1C3.His⋅SOD⋅cTra10 A-D
2. pSB1C3.His⋅SOD⋅cTAT A-D
3. pSB1C3.His⋅SOD⋅cLMWP A-D

Standard colony PCR settings

• Elongation time: 1:30

Gel verification

1 % agarose, 120 V

Expected bands

1. 884 bp
2. 854 bp
3. 863 bp

Results
Varying band sized, with the following clones seeming correct:

1. A
2. D
3. B & C