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Team:Stockholm/5 July 2010
From 2010.igem.org
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|'''Sample''' | |'''Sample''' | ||
|'''DNA conc (ng/ul)''' | |'''DNA conc (ng/ul)''' | ||
| - | |'''A | + | |'''A<sub>260</sub>/A<sub>280</sub>''' |
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|pSB1A3.BBa_J04450 | |pSB1A3.BBa_J04450 | ||
Revision as of 12:58, 6 July 2010
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Contents |
Andreas
Plasmid preparations
Prepared plasmids from ON cultures.
DNA concentrations
| Sample | DNA conc (ng/ul) | A260/A280 |
| pSB1A3.BBa_J04450 | 66.8 | 1.74 |
| pSB1C3.BBa_J04450 | 36.5 | 1.87 |
| pSB1AC3.BBa_J04450 | 41.0 | 1.95 |
| pSB1AK3.BBa_J04450 | 10.8 | 2.64 |
| pMA.BBa_J18930 | 33.3 | 1.95 |
| pMA.BBa_J18931 | 52.5 | 1.92 |
| pMA.BBa_J18932 | 34.1 | 1.99 |
Part and primer design
I investigated Mimmi's primers, most specifically the ones designed for ligation of our CPPs (TAT, LMWP and Transportan 10). Me and Johan discussed whether we should order them as is, or if we should instead have the genes synthesized. We decided that we should rethink the matter until tomorrow, when we can have a group meeting and discuss this further.
Johan also discovered that several of our genes contain disallowed BioBrick restriction sites:
- Tyrosinase contains one NgoMIV site at 500 bp.
- yCCS contains one EcoRI site at 230 bp.
- bFGF contains one AgeI site at 350 bp.
These need to be removed. We will discuss this tomorrow.
